| Theseus, Duke of Athens | John Anastasio |
| Hippolyta, Queen of the Amazons | Susan Tapper |
| Philostrate, Master of the Revels | Bob Moore |
| Egeus, Father of Hermia | Howard Goldstein |
| Hermia, Beloved of Lysander | Laurie Huntsman |
| Lysander, A Young Gentleman | Tom Curbishley |
| Demetrius, A Young Gentleman | Gregg Klein |
| Helena, Once Loved by Demetrius | Chrissy Lucash |
| Peter Quince (Prologue) | Lee Harrod |
| Nick Bottom (Pyramus) | Tom Moffit |
| Francis Flute (Thisby) | Fred Walker |
| Robin Starveling (Moonshine) | Gene Davidson |
| Tom Snout (Wall) | Dom DePhilippis |
| Snug (Lion) | Corey Nathan |
| Puck, also called Robin Goodfellow | John Erath III |
| Fairy (Cobweb) (Song) | Nurith Aizenman |
| Oberon, King of the Fairies | Stephen Kazakoff |
| Titania, Queen of the Fairies | Carol Kehoe |
| Fairy (Peaseblossom) | Christina Ennis |
| Fairy (Mustardseed) | John LaVigne |
| Fairy (Moth) | Bryan Grove |
| Boy | Jesse Neider |
| Dog | Roxanne |